5% (two-sided 95% CI should not exceed 5.5% to each direction from a point value), the sample size of 317 children would be sufficient to perform the tasks of the study protocol. Taking into account the possibility of patient or data loss of about

BTK inhibitor 10–15% it was planned to enroll 350 children into the main study group. The data of all 350 children were used in the final analysis. To reach the power of at least 80% with α-error of 0.05 the sample size for the laboratory part of the study was calculated based on the reference laboratory values for all the parameters and estimated minimal group values difference of 13.0 mcg/l for ferritin and 3.0 g/l for hemoglobin. To meet such estimations, the sample should include at least 92 persons. Taking into account the possibility that about 5% of the data see more could be lost, we included into the study 105 children randomly selected from the main study group. At the time of enrollment into the study 12 (19.05%) infants, 18 (11.69%)

children of the second and 2 (1.5%) children in the third year of life were breastfed. Thirty-five (55.56%), 63 (40.91%) and 24 (18.05%) children respectively in the three age groups were fed with infant (special) formula (Fig. 1). The diet composition was mostly adequate for age at the time of enrollment into the study (Tab. I). Thirty-two (9.14%) babies were breastfed and 122 (34.86%) children received infant formula. Two hundred and fifty-seven

(78.83%) children consumed infant cereals, 315 (93.47%) – beef, 191 (60.06%) – pork, 315 (91.3%) – poultry, 301 (87.76%) – fish, 314 (91.81%) – eggs, 322 (94.15%) – cheese, 342 (99.71%) – fruit and 343 (99.71%) – vegetables. However, the consumption of unmodified cow’s milk ranged from 60% in infants to 8% of children in the third year of life. The proportions of children who ate sweets or candy (48%), chocolate (33%), nuts (72%), as well as hot dogs and sausages (34%) were also significant (Tab. I). The average frequency of weekly formula consumption decreased with age, while the number of cow’s milk intakes increased. Infant cereals, vegetables and fruits remained most commonly used food to all ages. The daily diet of the majority of children contained these products. Older children D-malate dehydrogenase consumed more meat of all kinds, and the corresponding positive trend was particularly evident for pork. The amount of fish intake per week remained mostly unchanged. A similar conclusion could be drawn regarding the consumption of eggs and cheese. The frequency of use of “adult” products (ketchup, sauces, mayonnaise, etc.) increased with age. According to history data 59 (93.65%) infants, 149 (93.65%) children of the second and 125 (93.98%) children in the third year of life were breastfed at the study point or in the past. The average duration of breastfeeding was 10.