3 and >4.3 log IU/ml found in 26%, 48% and 26%, respectively. Prevalence of HBV variants was significantly
related to: sex (p=0.02), male were more likely to have BCP and BCP+PC; age (p=0.01), WT associated with younger patients; ethnicity (p<.001) WT and BCP found in Caucasians (51% and 49%), PC and BCP+PC found more frequently in black African (55% and 43%); HBV genotypes (p<.0001) WT and BCP found in genotype A (43% and 52%) while PC and BCP+PC found in D (32% and 36%) and E (50% and 41%) ; fibrosis stage (p<.0001) BCP, BCP+PC were associated with F>1 (52% and 50%) and WT and PC associated with F<1 (81% and 76%), HBsAg titers (p<.0001) WT associated with >4.3 log IU/ml (63%) while BCP, PC and BCP+PC associated with a titer <4.3logIU/ml (82%, 77%, 83%), HBV DNA levels (p<.0001) WT associated with a level >4.3 log IU/ml (73%) while PC associated with a level <3 .3log IU/ml (37%), In multivariate
analysis HBV variants were significantly click here and independently associated: WT with e(+) (p<.002) and high HBsAg titer (p<.01); BCP with more severe fibrosis (F >1) (p<.001); BCP+PC with more severe fibrosis (F >1)(p<.002), e(-) (p<.0001), genotypes D (p<.01) and E (p<.0001). Conclusions: PC and BCP+PC variants found more frequently in e(-) status. Patients with BCP and BCP+PC variants were more likely to have more severe fibrosis (F >1). We confirm a strong correlation between HBV variants and HBV genotypes independently from ethnicity and IL28B genotypes. Disclosures: Nathalie Boyer – Board Membership: MSD, find protocol JANSSEN; Speaking and Teaching: BMS Tarik Asselah – Consulting: BMS, Boehringer-Ingelheim, Roche, Merck-Schering Plough, Gilead, Janssen Patrick Marcellin
– Consulting: Roche, Gilead, BMS, Vertex, Novartis, Janssen-Tibotec, MSD, Boehringer, Pfizer, Abbott, Alios BioPharma; Grant/Research Support: Roche, Gilead, BMS, Novartis, Janssen-Tibotec, MSD, Alios BioPharma; Speaking and Teaching: MCE Roche, Gilead, BMS, Vertex, Novartis, Janssen-Tibotec, MSD, Abbott The following people have nothing to disclose: Michelle Martinot-Peignoux, Mar-tine Lapalus, Cédric Laouénan, Ana Carolina Cardoso, Roberto J. Carvalho-Filho, Ahmed El Ray, Simon Gosset Background and Aim: Replication of the hepatitis B virus (HBV) DNA genome proceeds via an RNA pregenome (HBV RNA), transcribed from cccDNA present in the nuclei of infected hepatocytes. Treatment of HBV infection with nucleos(t)ide analogues (NUC) suppresses HBV DNA synthesis by blocking reverse transcription, but does not affect HBV RNA synthesis. We hypothesized that during NUC therapy HBV pregenomes continue to be incorporated in viral particles and subsequently are secreted into the bloodstream. For this, we developed a sensitive assay to measure HBV RNA in plasma. Patients and Methods: HBV RNA (see below), HBV DNA (COBAS TaqMan – Roche), and HBsAg (Architect – Abbott) were measured in plasma of 10 chronic hepatitis B patients (5 HBeAg-positive and 5 negative) who received NUC therapy (7 entecavir and 3 tenofovir).