1H Nuclear Magnetic Resonance (NMR) metabolite profiling of faeces and urine samples Overall,
1H NMR results confirmed the trends and the major differences found between T-CD and HC samples through GC-MS/SPME analysis. Besides, other metabolites were found (Table 4). Try, Pro, Asn, His, Met, trimethylamine-N-ox and tyramine were higher in faecal samples of T-CD than HC children. By comparing the spectra of urine samples, median values of Lys, Arg, creatine and methylamine were higher than in T-CD children. On the contrary, median values of carnosine, glucose, glutamine and Selleckchem MEK inhibitor 3-methyl-2-oxobutanoic acid were the highest in HC children. Table 4 Median values and ranges of the relative concentration (‰) of organic compounds of faecal and urine samples from treated celiac disease (T-CD) children and non-celiac children (HC) as determined by 1H nuclear magnetic resonance (NMR) spectroscopy analysis Chemical class Treated celiac disease (T-CD) children Non-celiac children (HC) Median Range Median Range Faeces Tryptophane 1.13a 0.29 – 1.38 0.68b 0.19 – 1.33 Proline 2.74a 0 – 19.68 1.87b 0.71 – 6.47 Trimethylamine-N-ox click here 3.36a 1.16 – 11.60 1.82b 0.46 – 10.94 Histidine 5.56a 3.05 – 19.95 2.89b 0.93 – 11.03 Asparagine 2.01a 1.02 – 2.75 1.21b
0.51 – 2.17 Tyramine 2.81a 1.34 – 3.21 1.88b 0.74 – 7.87 Methionine 1.78a 0.99 – 3.30 1.50a 0.64 – 2.06 Urines Selleck RG7112 Carnosine 0.28b 0.12 – 0.48 0.43a 0.22 – 1.37 Glucose 14.66b 4.80 – 31.00 19.76a 15.33 – 53.73 Creatinine 38.51a 15.83 – 83.23 21.31b 10.40 – 61.80 Methylamine 1.45a 0.80 – 7.72 0.93b 0.32 – 2.36 Glutamine 4.05b 1.72 – 8.03 5.65a 3.14 – 8.55 Lysine-Arginine 8.96a 4.07 – 25.72 7.10b 5.59 – 11.08 Ornithine 1.87a 0.09 – 23.40 1.17a 1.03 – 2.08 3-Methyl-2-oxobutanoic acid 1.84b 1.12 – 2.60 2.35a 1.63 – 2.78 Data are the means of three independent experiments (n = 3) for each children. a-bMeans within a row with different superscript letters are significantly different (P < 0.05).
Discussion This study used culture-independent and culture-dependent methods and metabolomics analyses to investigate the differences in the microbiota and metabolome of 19 treated celiac disease (T-CD, under remission since 2 years) children and 15 non-celiac children (HC). The present study Nutlin-3 ic50 showed that the whole eubacterial community significantly changed between the duodenal microbiota of T-CD and HC children. In agreement, other authors [9] reported similar results when faecal samples of CD children were compared to those of HC. This result was surprising since an heterogeneous group like the ‘healthy controls’ should have more heterogeneity in DGGE microbial profiles. However, also Schippa et al [26] showed a peculiar microbial TTGE profile and a significant higher biodiversity in CD pediatric patients’ duodenal mucosa after 9 months of GFD compared to healthy control.