, HS1165: 36. Benson G: Tandem repeats finder: a program to analyze DNA sequences. Nucl Acids Res 1999,27(2):573–58.PubMedCrossRef 37. Peakall R, Smouse P: GENALEX 6: Genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 2006, 6:288–295.CrossRef

38. Raymond M, Rousset F: GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 1995, 86:248–249. 39. Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular evolutionary genetics analysis (MEGA) software Version 4.0. Mol Biol Evol 2007,24(8):1596–1599.PubMedCrossRef 40. Pritchard J, Stephens M, Donnelly P: Inference of population structure using multilocus genotype data. Genetics 2000, 155:945–959.PubMed 41. Jakobsson M, Rosenberg NA: CLUMPP: a cluster matching and permutation program for dealing with label switching and multimodality in analysis of population AP26113 nmr structure. Bioinformatics 2007, 23:1801–1806.PubMedCrossRef 42. Rosenberg NA: DISTRUCT: a program for the graphical display of population structure. Mol Ecol Notes (2004) 2004, 4:137–138.CrossRef Authors’ contributions HL, MSI, JMG, YPD, HDC, GK and ELC coordinated the study, collected Doramapimod nmr samples

and provided preliminary data. HL, JMG, and YB carried out genotyping of HLB samples. MSI, JMG and HL analyzed results and wrote the paper. All authors read and approved the final manuscript.”
“Background The zebrafish (Danio rerio) is a small tropical teleost that bridges the phylogenetic evolutionary Rebamipide gap between invertebrates and mammals

in experimental biomedicine. It is evolutionarily closer to humans than fruit flies and nematodes, and is easier to work with and study than mice [1]. Recently, increased interest in using zebrafish for studies of human diseases as disparate as cancer, microbial infections and immune-pathological changes has evolved [2]. As an infection model, zebrafish have been employed for study of both human and fish pathogens [1, 3–6]. Aeromonas hydrophila is a ubiquitous Gram-negative aquatic bacterium and opportunistic pathogen causing fatal hemorrhagic septicemia in several fish species including warm water and temperate aquaculture species [7–9]. In particular, A. hydrophila infections have been repeatedly reported from zebrafish facilities causing unusual [10] and sometimes high mortality rates [11]. Some strains of A. hydrophila have also been reported to be important human pathogens [12]. Conjugative R plasmids assigned to the IncU incompatibility group are widespread in environmental and fish pathogenic Aeromonas species worldwide [13]. An IncU representative, pRAS1, was detected in Aeromonas salmonicida from Norway [14]. This plasmid is very similar to an IncU plasmid derived from a human urinary tract pathogenic Escherichia coli in Eastern Germany as early as the 1970′s [15].