Immunogenicity of the recombinant protein was determined by injecting BALB/C mice. The antibody produced therein could efficiently recognize and bind ferric enterobactin binding protein. Immunized mice challenged with higher doses of selected bacteria survived.
Conclusions: Significant recognition by the antibody of ferric enterobactin binding protein may lead to its application in the restriction of Enterobacteriaceae propagation. (C) 2010 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.”
“Cortical kindling causes alterations within the motor cortex and results in long-standing LY2606368 motor deficits. Less attention has
been directed to other regions that also participate in the epileptiform activity. We examined if cortical kindling Could induce changes in excitatory and inhibitory receptor subunit mRNA in the amygdala/piriform regions and if such changes are associated with behavioral deficits. After cortical kindling, amygdala/piriform regions were dissected to analyze mRNA levels of NMDA, AMPA, and GABA receptor Subunits using reverse transcription polymerase chain reaction, or rats were subjected to a series of behavioral tests. Kindled rats had significantly greater amounts JQ1 purchase of GluR1 and GluR2 AMPA receptor
mRNA, and alpha 1 and alpha 2 GABA receptor subunit mRNA, compared with sham controls, which was associated with greater anxiety-like behaviors in the elevated plus maze and reduced freezing behaviors in the fear conditioning task. In Summary, cortical kindling produces dynamic receptor subunit changes in regions in addition to the seizure focus. (C) 2009 Elsevier Inc. All rights reserved.”
“Inclusion of nano-clays into hydrogels is an efficient approach to produce nanocomposite hydrogels. The introduction of nano-clay into hydrogels causes
an increase in water absorbency. In the present work, Nanocomposite hydrogels based on kappa-carrageenan were synthesized using sodium montmorillonite as nano-clay. Acrylamide and methylenebisacrylamide were used as monomer and crosslinker, respectively. The structure of nanocomposite hydrogels was investigated by XRD and SEM techniques. Swelling behavior of nanocomposite hydrogels selleck chemical was studied by varying clay and carrageenan contents as well as methylenebisacrylamide concentration. An optimum swelling capacity was achieved at 12% of sodium montmorilonite. The swollen nanocomposite hydrogels were used to study water retention capacity (WRC) under heating. The results revealed an increase in WRC due to inclusion of sodium montmorilonite clay. (C) 2010 Wiley Periodicals, Inc. J Appl Polym Sci 118: 2989-2997,2010″
“Sex steroids can influence seizures. Estrogen (E(2)), progesterone (P(4)), and its metabolite, 5 alpha-pregnan-3 alpha-ol-20-one (3 alpha,5-alpha THP), in particular, have received much attention for exerting these effects.