Moreover, the embryonic stem cell platform, exposed the key subpopulations of ovarian cancer stem cells – which are believed to be the most important target for a sustained response with anti-cancer therapy. These subpopulations show the capacity for both self-renewal and tumorigenic differentiation in a niche-dependent manner, and are characterized by the expression of specific markers for cancer stem cells. This study underscore the potential experimental utility of the hESC-derived cellular
Luminespib price microenvironment to expose certain cancer cell sub-populations that do not grow into a tumor in the conventional direct tumor xenograft platform and therefore are most probably not readily accessible to characterization and testing of anticancer therapies. O151 Hepatomimetic
Properties of Colon Cancer Cells: Microenvironmental Regulation and Clinical Implications Combretastatin A4 chemical structure Fernando Vidal-Vanaclocha 1 , Javier Beaskoetxea2, Naiara Telleria2, Amaia Del Villar2, Andrés Valdivieso3, Jorge Ortiz de Urbina3 1 Department of Cell Biology and Histology, Basque Country University School of Medicine, Leioa, Bizkaia, Spain, 2 Pharmakine SL, Derio, Bizkaia, Spain, 3 Hepatobiliar Tumor Surgery Sevice, Cruces Hospital, Cruces-Baracaldo, Bizkaia, Spain Organ-specific colonization of cancer cells is an important feature of metastasis and it has been MK0683 reported that distinct alterations in gene expression underlie metastasis to defined organs. However, the regulation and clinical projection of this tropism are unknown. DNA microarrays and RT-PCR were used to determine the gene expression profile of hepatic colorectal carcinoma metastases and tumor-unaffected liver tissue from same patients. HT-29 human colon carcinoma and primary cultured human hepatocytes and liver myofibroblasts were used to determine if both tumor and liver cells are mutually influencing their expression of metastasis-associated genes. Three microenvironment-related
gene expression categories were detected: 1) Hepatic metastases genes not expressed by tumor-unaffected liver tissue. Some of them were already expressed at primary tumors of patients having hepatic colon carcinoma metastases in less than five years, and were expressed by both HT-29 cells given selleck products cultured liver cell-conditioned media (CM) and liver cells given HT-29 cell-CM. 2) Genes co-expressed by hepatic metastases and tumor-unaffected liver tissue. These were not expressed by primary tumors. This category also included both liver-specific genes expressed by HT-29 cells given liver cell-CM, and colon cancer-specific genes expressed by liver cells receiving HT-29-CM. 3) Genes of tumor-unaffected liver tissue not expressed at hepatic metastases. These were expressed by liver cells, but not by colon cancer cells, and represented the genetic background of the hepatic metastasis microenvironment.