PLoS Biol 2007, 5: 2177–2189.PubMedCrossRef 73. Lupp C, Robertson ML, Wickham ME, Sekirov

I, Champion OL, Gaynor EC, Finlay BB: Host-mediated inflammation disrupts the intestinal AR-13324 cost microbiota and promotes the overgrowth of Enterobacteriaceae . Cell Host Microbe 2007, 2: 119–129.PubMedCrossRef 74. Artis D: Epithelial-cell recognition of commensal bacteria and maintenance of immune homeostasis in the gut. Nat Rev Immunol 2008, 8: 411–420.PubMedCrossRef 75. Kaser A, Zeissig S, Blumberg RS: Inflammatory bowel disease. Annu Rev Immunol 2010, 28: 573–621.PubMedCrossRef 76. Baron JH, Connell AM, Lennard-Jones JE: Variation between observers in describing mucosal appearances in proctocolitis. BMJ 1964, 5375: 89–92.CrossRef 77. DeSantis TZ, Hugenholtz P, Larsen N, Rojas M, Brodie EL, Keller K, Huber T, Dalevi D, Hu P, Andersen GL: Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB. Appl Environ Microbiol 2006, 72: 5069–5072.PubMedCrossRef 78. Ludwig W, Strunk O, Westram R, Richter L, Meier H, Yadhukumar, Buchner A, Lai

T, Steppi S, Jobb G, Förster W, Brettske I, Gerber S, Ginhart AW, Gross O, Grumann S, Hermann S, Jost R, König A, Liss T, Lüssmann R, May M, this website Nonhoff B, Reichel B, Strehlow R, Stamatakis A, Stuckmann N, Vilbig A, Lenke M, Ludwig T, et al.: ARB: a software environment for sequence data. Nucleic Acids Res 2004, 32: 1363–1371.PubMedCrossRef 79. Ashelford KE, Chuzhanova NA, Fry JC, Jones AJ, Weightman AJ: New screening software shows that most recent large 16S rRNA gene Adenylyl cyclase clone libraries contain Capmatinib molecular weight chimeras. Appl Environ Microbiol 2006, 72: 5734–5741.PubMedCrossRef 80. Ashelford KE, Chuzhanova NA,

Fry JC, Jones AJ, Weightman AJ: At least 1 in 20 sequence records currently held in public repositories is estimated to contain substantial anomalies. Appl Environ Microbiol 2005, 71: 7724–7736.PubMedCrossRef 81. Schloss PD, Handelsman J: Introducing DOTUR, a computer program for defining operational taxonomic units and estimating species richness. Appl Environ Microbiol 2005, 71: 1501–1506.PubMedCrossRef 82. Johnson M, Zaretskaya I, Raytselis Y, Merezhuk Y, McGinnis S, Madden TL: NCBI BLAST: a better web interface. Nucleic Acids Res 2008, (36 Web server) : W5-W9. 83. Letunic I, Bork P: Interactive Tree Of Life (iTOL): an online tool for phylogenetic tree display and annotation. Bioinformatics 2007, 23: 127–128.PubMedCrossRef 84. Nadkarni MA, Martin FE, Jacques NA, Hunter N: Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set. Microbiology 2002, 148: 257–266.PubMed Authors’ contributions AWW carried out the clone library construction, performed the sequence analysis and drafted the manuscript. CC co-ordinated the sequencing.