The most common mutation in cirrhosis patients was located in the c-terminus of the TERT gene (the c.3325G>A mutation resulting in the amino acid change p.G1109R) (Fig. 2B, Table 1, Supporting Fig. 1G). This mutation is located next to an amino acid change (p.T1110M), which has previously

been associated with pulmonary fibrosis.21 The mean age of the patients with telomerase mutation was 55.8 years (Table 2) compared to a mean age of www.selleckchem.com/products/azd2014.html 58.7 years in the group of cirrhosis patients without telomerase mutations. There was no obvious overrepresentation of a specific disease etiology or ethnicity in the mutation carriers compared to the controls (see above). However, many of the mutation carriers

showed a rapid progression of chronic liver disease toward cirrhosis of liver cancer development (Table 2). Specifically, the frequency of endstage liver disease (defined as Child B or C cirrhosis, hepatocellular carcinoma [HCC] occurrence, and conduction or evaluation for liver transplantation) was significantly higher in the group of cirrhosis patients harboring telomerase mutations (93%, 13 out of 14) compared to cirrhosis patients without Selleckchem ZVADFMK telomerase mutations (62%, 327 out of 507, P = 0.042), indicating that telomerase mutations may induce a more aggressive progression of chronic liver disease—a hypothesis that should be addressed in future prospective trials. Cirrhosis patients carrying telomerase gene mutations had significantly shorter telomeres in peripheral blood cells compared to control patients without telomerase gene mutations (Fig.

3A, P = 0.0004). A significant reduction of telomerase activity was detectable by TRAP assay for three of the investigated, cirrhosis-associated telomerase mutations (p.P65A: P < 0.0001, p.G1109R: P = 0.0035, and p.P380S: P < 0.0001), including the most frequent mutation p.G1109R (Fig. 3B,C). Studies on telomerase-negative, human fibroblasts revealed that the cirrhosis-associated TERT mutations (p.P65A and p.G1109R) did not lose immortalization capacity when overexpressed (Fig. 3D). However, proliferation rates of fibroblast lines expressing these TERT mutants were significantly 上海皓元医药股份有限公司 reduced compared to cells expressing wildtype TERT (Fig. 3D). Similarly, Epstein-Barr virus-immortalized, primary lymphocytes from two patients with a homozygous p.G1109R TERT mutation showed an impaired proliferation capacity (Fig. 3E) and shortened telomeres (Fig. 3F,G) compared to immortalized lymphocytes from cirrhosis patients with wildtype TERT. γH2Ax staining of liver sections of six cirrhosis mutations carriers and eight liver cirrhosis patients without telomerase mutation did not reveal any significant difference in the percentage of γH2Ax-positive hepatocytes, a marker for the induction of DNA double-strand breaks (data not shown).