The ovarian donors were transgenic mice carrying FVB/N-Tg
(PolII-luc) as a reporter gene, encoding luciferase to catalyse luciferin which results in visible light emission as bioluminescence. There were three groups of recipients: (i) group A: BALB/c mice without immunosuppressant treatment; (ii) group B: BALB/c mice receiving a cocktail immunosuppressant treatment; and (iii) group C: immunodeficient NOD-SCID mice without immunosuppression. Luciferin BLI was used to follow graft survival, and viable follicle numbers were counted as a measure of success. Bioluminesence intensity fluctuated www.selleckchem.com/products/PLX-4720.html but was consistent with the end-point counts of viable follicle numbers. Group A showed loss of viable follicles and bioluminesence disappeared as early as day 10 following ovarian engraftment, indicating strong immune rejection. Groups B and C showed graft survival and measurable bioluminesence for up to 30 days. In conclusion, BLI provided non-invasive longitudinal dynamic monitoring of ovarian grafts with excellent sensitivity and spatial resolution. This approach should prove valuable for research on ovarian transplantation. (C) 2010, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.”
“Purpose Facet tropism is defined as asymmetry between left and right facet joints and is postulated as a possible cause of
disc herniation. In the present study, the authors used a 3-T MRI to investigate the association between facet tropism and lumbar disc herniation at this website a particular motion segment.
They also examined whether the disc herniated towards the side of the more coronally oriented facet joint.
Methods Sixty patients (18-40 years) with single level disc herniation (L3-L4, L4-L5, or L5-S1) were included in the study. Facet angles https://www.sellecn.cn/products/btsa1.html were measured using MRI of 3-T using the method described by Karacan et al. Facet tropism was defined as difference of 10 degrees in facet joint angles between right and left sides. Normal disc adjacent to the herniated level was used as control. We also examined if disc herniated towards the side of more coronally oriented facet.
Results Twenty-five herniations were at L4-L5 level and 35 at L5-S1. Statistical analysis was performed using the Fischer Exact Test. At L4-L5 level, 6/25 cases had tropism compared to 3/35 controls (p = 0.145). At L5-S1 level, 13/35 cases had tropism as compared to 1/21 controls (p = 0.0094). Of 19 cases having tropism, the disc had herniated towards the coronally oriented facet in six (p = 0.11).
Conclusion The findings of the study suggest that facet tropism is associated with lumbar disc herniation at the L5-S1 motion segment but not at the L4-L5 level.”
“LH plays a key role in the intermediate-late phases of folliculogenesis. Although ovarian stimulation is efficiently achieved in most cases by the administration of exogenous FSH alone, specific subgroups of women may benefit from LH activity supplementation during ovarian stimulation.