The PCR products are 250 bp for EYA4 (A) and 540 bp for β-actin (B). Table 2 Rates of detection of EYA4 and hTERT mRNA in peripheral blood mononuclear cells of the study subjects Control subjects
(n = 50) BCH (n = 50) ESCD (n = 50) ESCC (n = 50) EYA4 ≥ 0.2, n(%) 7(14.0) 10(20.0) 13(26.0) 26(52.0) LY3023414 solubility dmso < 0.2, n(%) 43(86.0) 40(80.0) 37(74.0) 24(48.0) hTERT ≥ 0.8, n(%) 12(24.0) 15(30.0) 26(52.0) 40(80.0) < 0.8, n(%) 38(76.0) 35(70.0) 24(48.0) 10(20.0) ×:χ2 = 19.643, P < 0.001, and linear-by-linear association = 16.246, P < 0.001 for EYA4 mRNA BMN 673 mw expression in the four groups; χ2 = 69.149, P < 0.001 and linear-by-linear association = 41.994, P < 0.001 for hTERT mRNA expression in the four groups. BCH, Basal cell hyperplasia; ESCD, esophageal squamous cells dyspalsia; ESCC, esophageal squamous cells cancer. As shown in Table 2, the band intensity ratios of EYA4 mRNA with a β-actin positive cut-off value of ≧ 0.2 indicated that EYA4 mRNA expression increased progressively according to
the severity of the pathology: controls 14.0% (7/50), BCH 20.0% (10/50), ESCD 26.0% (13/50), and ESCC 52.0% (26/50). There was a significant linear-by-linear association of the four groups. The band intensity ratios LCZ696 clinical trial of hTERT mRNA with a β-actin positive cut-off value of ≧ 0.8 indicated that hTERT mRNA expression also increased with the progressively severity of the disease, and the positive expression rates in the four groups were 24% (12/50), 30.0% (15/50), 52% (26/50) and 80% (40/50), respectively. The Spearman correlation coefficient of hTERT and EYA4 mRNA expression in peripheral blood mononuclear cells and in the tissues was 0.80 (P < 0.01). This indicated that the expression of the two markers in peripheral blood click here mononuclear cells was accurate. As shown in Table 3, multinomial logistic regression
analysis gave odds ratios (ORs) for EYA4 and hTERT mRNA expression also increased with the severity of the diseases after adjustment for age, gender, smoking index, drinking index and family history of esophageal cancer. However, only the OR value of the EYA4 mRNA expression in ESCC group was significant. Table 3 Association of the expression of EYA4 and hTERT mRNA in peripheral blood mononuclear cells with esophageal diseases BCH (n = 50) ESCD (n = 50) ESCC (n = 50) EYA4 mRNA OR(95%CI) 1.32(0.47-3.66) 1.85(0.69-4.94) 5.69(2.23-14.53) OR(95%CI)+ 1.90(0.62-5.81) 1.72(0.54-5.45) 5.07(1.56-16.52) hTERT mRNA OR(95%CI) 0.90(0.33-2.45) 1.18(0.42-3.36) 2.03(0.63-6.55) OR(95%CI)+ 1.10(0.37-3.26) 1.12(0.34-3.72) 2.87(0.63-13.07) +: OR(95%CI) was adjusted for age, smoking, drinking, income and family history of esophageal carcinoma. BCH, Basal cell hyperplasia; ESCD, esophageal squamous cells dyspalsia; ESCC, esophageal squamous cells cancer.