This research was financially supported by grants from the CYTED (AGROSEQ; 107PIC0312), Spanish Ministerio de Ciencia e Innovación (BIO2011-22833), Spanish National Network on Extremophilic Microorganisms (BIO2011-12879-E), and Junta de Andalucía (P08-CVI-03724). Mercedes

Reina-Bueno was recipient of a fellowship from the Spanish Ministerio de Ciencia e Innovación. Montserrat Argandoña holds a postdoctoral contract from Junta de Andalucía. ��-Nicotinamide datasheet Electronic supplementary material Additional file 1: Table S1. R. etli genes involved in trehalose and glutamate metabolis. (PDF 68 KB) Additional file 2: Figure S1. Genomic analysis of R. etli pathways involved in trehalose metabolism. (A) Genomic context of genes involved in trehalose metabolism. Position and clustering of genes included in Additional file 1: Table S1. are indicated. (B) Neighbor-joining

tree based on proteins belonging to families 13 and 15 of glycosydases, including the three TreC-like proteins from R. etli. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The E. coli and Rhrodothermus marinus representatives were used as outgroup. The evolutionary distances were computed using the Poisson correction method and are in the units of the number of amino acid substitutions per site. The rate variation check details among sites was modeled with a gamma distribution (shape parameter = 1). All positions containing gaps and missing data were eliminated from the dataset (complete deletion option). Bootstrap probabilities (as percentage) were determined Isotretinoin from 1000 resamplings. (PDF 34 KB) Additional file 3: Figure S2. Growth of R. wild type (WT) and the otsAch mutant CMS310 with trehalose and glucose as the sole carbon source. Cells were grown in at 28°C in B- minimal medium with 20 mM trehalose or glucose and 0.0 or 0.2 M NaCl. (PDF 80 KB) References 1. Miller KJ, Wood JM:

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