The values of specific volume found (2.57–4.05 cm3/g) corroborate with values found for bread made of wheat flour and cassava, with 30 min fermentation

(Shittu et al., 2007). The Control presented specific volume of 4.01 cm3/g. None of the samples reached the ERK inhibitor mw specific volume ideal for white pan bread, of 6 cm3/g, mentioned in the literature (Kim, Steel, & Chang, 2005), possibly due to the shorter fermentation time used. Tthe mathematical model (R2 = 0.95; Fcalc/Ftab = 16) obtained for the dependent variable of specific volume is shown in Equation (3). equation(3) Specificvolume(cm3/g)=2.99−0.49MO+0.13MO2 It is observed that the increase of the concentration of MO caused a reduction of the specific volume of the bread, and that the addition of RE, within the range studied, did not interfere in this response. According to Serna-Saldivar, Zorrilla, La Parra, Stagnitti, and Abril

(2006), white pan bread enriched with 1.6 g/100 g or 3.2 g/100 g (flour basis) of microcapsules of oil rich in DHA (20 g/100 g oil) presented reduction in the volume HKI 272 when compared to that which was produced with different sources of omega-3 fatty acids (oils and emulsions). The addition of microcapsules can dilute the gluten, due to the composition of wall material, interfering with the retention of gases during the baking process. The firmness values varied from 4.56 to 13.81 N. The Control presented firmness of 5.50 N. The mathematical model (R2 = 0.88; Fcalc/Ftab = 12.46) for the dependent variable of firmness, determined by instrumental texture analysis, is shown in Equation (4). equation(4) Firmness(N)=8.73+6.15MOFirmness(N)=8.73+6.15MO It is observed that increasing tuclazepam the concentration

of MO caused a linear increase in the firmness of the bread, and the addition of RE, within the range studied, also did not interfere in this response. Comparing the surfaces obtained for specific volume and firmness, it is observed that bread with lower specific volume is firmer. White pan bread enriched with 1.6 g/100 g and 3.2 g/100 g of microcapsules of oil rich in DHA presented inferior texture characteristics in comparison to bread made with different sources of omega-3 (Serna-Saldivar et al., 2006). The moisture content of the crumb has some mechanical and qualitative implications, being related with the gelatinization of starch in the dough during the baking process and correlated with crumb softness (Zghal et al., 2002). The crumb moisture contents presented values from 36.77 to 41.86 g/100 g. The Control had a moisture content of 38.23 g/100 g. It was not possible to obtain a mathematical model and response surface to describe the behavior of this investigated variable because R2 was inferior to 0.70. This indicates that the variation of the MO and RE, within the ranges studied, had no effect on crumb moisture.